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Mouse ChemR23 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7610G

R&D Systems, part of Bio-Techne
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FAB7610G

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 477806

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with mouse ChemR23

Specificity

Detects mouse ChemR23 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse ChemR23 Alexa Fluor® 488-conjugated Antibody

Detection of ChemR23 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of ChemR23 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse ChemR23 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7610G, filled histogram) or isotype control antibody (IC013G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of ChemR23 in HEK293 cells transfected with Human ChemR23 by Flow Cytometry

HEK293 cells transfected with Human ChemR23 were stained with Rat Anti-Mouse ChemR23 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7610G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse ChemR23 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line; HEK293 cells transfected with Human ChemR23
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ChemR23

ChemR23 is a chemoattractant receptor expressed on dendritic cells and inflammatory M1 macrophages. ChemR23 binds Chemerin and Resolvin E1 (a lipid mediator), and promotes inflammation resolution. ChemR23 also functions as a coreceptor for SIV and some primary HIV-1 strains (1,2). Mouse and human ChemR23 share 80% amino acid sequence identity.

References

  1. Samson, M. et al. (1998) Eur. J. Immunol. 28:1689.
  2. Herova, M. et al. (2015) J. Immunol. 194:2330.

Long Name

Chemokine-like Receptor 1/Orphan G Protein-coupled Receptor ChemR23

Alternate Names

cmklr1, Dez, Gpcr27

Entrez Gene IDs

1240 (Human); 14747 (Mouse); 60669 (Rat)

Gene Symbol

CMKLR1

Additional ChemR23 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse ChemR23 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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