Mouse COCO Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3356
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Arg24-Leu185
Accession # Q76LW6
Specificity
Clonality
Host
Isotype
Applications for Mouse COCO Antibody
Western Blot
Sample: Recombinant Mouse COCO (Catalog # 3356-CC)
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: COCO
COCO, also known as DAND5, Dante, and CKTSF1B3, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and Cerberus (DAND4). DAN family members contain a cysteine knot domain that is homologous to that found in other TGF-beta superfamily ligands such as BMPs that play important roles in tissue morphogenesis and developmental processes (1‑6). The mouse COCO cDNA encodes a 185 amino acid (aa) precursor with a 23 aa signal sequence (7, 8). COCO has eight Cys residues in the cysteine knot which places it in the CAN (or eight-membered ring) subfamily of BMP antagonists along with the other DAN family proteins (1). Mature mouse COCO shares 62% and 27% aa sequence identity with human and Xenopus COCO, respectively. It shares 22%‑27% aa sequence identity with mouse DAN, Gremlin, PRDC, and Cerberus. In Xenopus embryos, COCO is expressed by pluripotent ectodermal cells. Expression is abruptly downregulated prior to gastrulation, and the loss of ectodermal cell pluripotency is coincident with COCO downregulation (7). COCO is required for Xenopus left-right axis formation (9). It functions predominantly on the right side of the embryo, although it is equally expressed on both left and right sides (9). COCO binds and inhibits activin, BMP-4, GDF-3/derrière, Wnt8, and Xnr1 (7, 9). In mouse, COCO expression is elevated on the right side of Henson’s node at the early somite stage, in contrast to the left side expression of Nodal (8).
References
- Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
- Katoh, M. and M. Katoh (2004) Oncol. Rep. 12:423.
- Katoh, M. and M. Katoh (2005) Int. J. Mol. Med. 15:885.
- Baron, M.H. (2005) Exp. Hematol. 33:1015.
- Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
- De Robertis, E.M. and H. Koruda (2004) Annu. Rev. Cell Dev. Biol. 20:285.
- Bell, E. et al. (2003) Development 130:1381.
- Pearce, J.J. et al. (1999) Dev. Biol. 209:98.
- Vonica, A. and A.H. Brivanlou (2007) Dev. Biol. 303:281.
Alternate Names
Gene Symbol
UniProt
Additional COCO Products
Product Documents for Mouse COCO Antibody
Product Specific Notices for Mouse COCO Antibody
For research use only