Mouse Collagen II Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3615
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse sternum-derived Collagen II
Specificity
Detects mouse Collagen II in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Collagen II Antibody
Collagen II in Mouse Chondrocytes.
Collagen II was detected in immersion fixed mouse mesenchymal stem cell-differentiated chondrocytes using 10 µg/mL Sheep Anti-Mouse Collagen II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3615) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse Collagen II Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse mesenchymal stem cell-differentiated chondrocytes
Sample: Immersion fixed mouse mesenchymal stem cell-differentiated chondrocytes
Western Blot
0.1 µg/mL
Sample: Mouse Collagen II
Sample: Mouse Collagen II
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF3615 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen II
Mouse Type II Collagen is a 100 kDa fibrillar collagen found in cartilage, vitreous body and vertebral disk. It is synthesized as a 1459 amino acid (aa) preproprecursor that contains an N-terminal and a C-terminal proregion. Intracellularly, type II Collagen homotrimerizes into a fibril and is then secreted. The proregions are then cleaved by procollagenases and the processed homotrimer covalently crosslinks to other type II fibrils. Mature mouse Collagen II shares 95% and 98% aa sequence identity with human and rat Collagen II, respectively.
Alternate Names
Chondrocalcin, COL2A1, SEDC
Gene Symbol
COL2A1
Additional Collagen II Products
Product Documents for Mouse Collagen II Antibody
Product Specific Notices for Mouse Collagen II Antibody
For research use only
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