J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse Complement Component C5a R1 APC-conjugated Monoclonal Antibody (Catalog # FAB6467A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Complement Component C5aR1 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Complement Component C5aR1

Complement Component C5a R1, also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5a R1 binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5a R1/C5a interactions are protective during allergen sensitization. Mouse C5a R1 shares 66% and 77% amino acid sequence identity with human and rat C5a R, respectively.

Long Name

Complement Component C5a Receptor 1

Alternate Names

C5AR1, CD88, Complement Component C5a R1

Entrez Gene IDs

728 (Human); 12273 (Mouse)

Gene Symbol

C5AR1

UniProt

P30993

Additional Complement Component C5aR1 Products

Product Documents for Mouse Complement Component C5aR1 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse Complement Component C5aR1 APC-conjugated Antibody

For research use only

Citations
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Flow Cytometry

Mouse Complement Component C5aR1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6467A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Complement Component C5aR1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Complement Component C5aR1 APC-conjugated Antibody

Detection of Complement Component C5a R1 in J774A.1 Mouse Cell Line by Flow Cytometry.