Mouse CRISP-4 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF5017
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CRISP-4
Leu23-Lys250
Accession # NP_084309
Leu23-Lys250
Accession # NP_084309
Specificity
Detects mouse CRISP-4 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse CRISP-1, -2, and -3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse CRISP-4 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CRISP-4
Sample: Recombinant Mouse CRISP-4
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRISP-4
CRISP‑4 (cysteine‑rich secretory protein 4) is a secreted, 31 kDa member of the CRISP family of proteins. In mouse, its expression is limited to the epididymis, seminal vesicle and vas deferens and it appears to bind to differentiating sperm. Mature mouse CRISP‑4 is 230 amino acids (aa) in length. It contains an SCP (sperm coating protein) region (aa 42‑178) followed by a C‑terminal CRISP domain (aa 196‑250). There appears to be an alternate start site four amino acids upstream of the standard start site. Over aa 22‑550, mouse CRISP‑4 shares 90% aa identity with rat CRISP‑4 and 64% aa identity with human CRISP‑1, the human ortholog to mouse CRISP‑4.
Long Name
Cysteine-rich Secretory Protein 4
Alternate Names
CRISP4
Gene Symbol
CRISP4
UniProt
Additional CRISP-4 Products
Product Documents for Mouse CRISP-4 Biotinylated Antibody
Product Specific Notices for Mouse CRISP-4 Biotinylated Antibody
For research use only
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