Cryptic was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse Cryptic Monoclonal Antibody (Catalog # MAB18401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Mouse Cryptic Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: D3 mouse embryonic stem cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cryptic

Cryptic, also known as CFC-1, is a member of the Epidermal Growth Factor-Cripto/FRL-1/Cryptic (EGF-CFC) family of signaling proteins. It is expressed during mesoderm differentation and is necessary for determining left-right asymmetry. Cryptic functions as a co-receptor that regulates signaling by some TGF-beta superfamily members.

Alternate Names

CFC-1, CFC1

Entrez Gene IDs

55997 (Human); 12627 (Mouse)

Gene Symbol

CFC1

UniProt

P97766

Additional Cryptic Products

Product Documents for Mouse Cryptic Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Cryptic Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Cryptic Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB18401

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Cryptic Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Cryptic Antibody

Cryptic in D3 Mouse Cell Line.