Mouse CXCL16 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB503

R&D Systems, part of Bio-Techne
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MAB503-100
MAB503-500
MAB503-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot

Cited:

ELISA Development, Flow Cytometry, Immunohistochemistry, In vivo assay, Luminex Development, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 142417

View all CXCL16 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCL16
Asn27-Pro114
Accession # Q8BSU2

Specificity

Detects mouse CXCL16 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, recombinant mouse CXCL1, 2, 6, 9, 10, 11, 12, 13, 14, 15, recombinant rat CXCL1, 2, 3, 5, or recombinant porcine CXCL8 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CXCL16 Antibody

Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody.

Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody.

Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL16 Chemokine Domain (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL16 Monoclonal Antibody (Catalog # MAB503). The ND50 is typically 0.15-0.6 µg/mL.

Applications for Mouse CXCL16 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize CXCL16-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CXCR6. Matloubian, M. et al. (2000) Nature Immunol. 1:298. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse CXCL16 Chemokine Domain.

Mouse CXCL16 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CXCL16 Biotinylated Antibody (Catalog # BAF503)
  • Standard: Recombinant Mouse CXCL16 Protein (Catalog # 503-CX)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB503 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL16

Mouse CXCL16 (CXC chemokine 16) is a non-ELR motif-containing CXC chemokine with a transmembrane domain. CX3CL1/Fractalkine andCXCL16 are the only two transmembrane chemokines within the superfamily. Mouse CXCL16 cDNA encodes a 246 amino acid (aa) precursor protein with a putative 26 aa signal peptide, an 88 aa chemokine domain, an 87 aa mucin-like spacer region, a 22 aa transmembrane domain, and a 23 aa cytoplasmic tail. Mouse and human CXCL16 share 49% aa sequence identity. Mouse CXCL16 is produced by dendritic cells in lymphoid organ T cell zones and bycells in the splenic red pulp both as membrane-bound and soluble forms. Based on northern blot analysis, CXCL16 is also expressed in some non-lymphoid tissues such as lung, small intestine and kidney. The receptor for CXCL16 has been identified as CXCR6 (previously called Bonzo, STRL33 or TYMSTR), which is also a coreceptor for HIV entry. CXCR6 is expressed on naive CD8 cells, natural killer T cells and activated CD8 and CD4 T cells.

Alternate Names

CXCL16

Entrez Gene IDs

58191 (Human); 66102 (Mouse)

Gene Symbol

CXCL16

UniProt

Q8BSU2

Additional CXCL16 Products

Product Documents for Mouse CXCL16 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse CXCL16 Antibody

For research use only

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