HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR1/IL-8 RA or (B) mouse CXCR2/IL-8 RB and eGFP was stained with Rabbit Anti-Mouse CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB8628). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CXCR1/IL-8RA Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse CXCR1/IL‑8 RA

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR1/IL-8RA

CXCR1, also known as IL-8 RA and CD181, is an approximately 60 kDa 7TM glycoprotein that functions as a receptor for the chemokine CXCL8/IL-8. It is expressed on neutrophils, monocytes, CD8 T cells, FoxP3+ CD4 Treg cells, mast cells, neuronal and glial cells, vascular endothelial cells, and melanoma. CXCR1 forms homodimers and heterodimers with CXCR2/IL-8 RB. It can be cleaved from neutrophils in the lungs of cystic fibrosis patients to release fragments that promote CXCL8 production from airway epithelial cells. CXCR1 mediates neutrophil activation and chemotaxis to sites of inflammation as well as angiogenesis and melanoma invasiveness. Mouse CXCR1 shares 65% and 89% amino acid sequence identity with human and rat CXCR1, respectively.

Long Name

Interleukin 8 Receptor A

Alternate Names

CD181, IL-8 RA, IL8RA

Entrez Gene IDs

3577 (Human); 227288 (Mouse); 54258 (Rat)

Gene Symbol

CXCR1

UniProt

Q810W6

Additional CXCR1/IL-8RA Products

Product Documents for Mouse CXCR1/IL-8RA Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CXCR1/IL-8RA Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CXCR1/IL-8RA Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8628

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all CXCR1/IL-8RA Antibodies »

Product Summary for Mouse CXCR1/IL-8RA Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CXCR1/IL-8RA Antibody

Detection of CXCR1/IL‑8 RA in HEK293 Human Cell Line Transfected with Mouse CXCR1/IL‑8 RA and eGFP by Flow Cytometry.