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Mouse CXCR6 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2145P

R&D Systems, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 221002

Product Specifications

Immunogen

CHO Chinese hamster ovary cell line transfected with mouse CXCR6
Met1-Leu351
Accession # Q9EQ16

Specificity

Stains mouse CXCR6-transfected cells but not irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CXCR6 PE-conjugated Antibody

Detection of CXCR6 antibody in BaF3 Mouse Cell Line Transfected with Mouse CXCR6 and eGFP antibody by Flow Cytometry.

Detection of CXCR6 in BaF3 Mouse Cell Line Transfected with Mouse CXCR6 and eGFP by Flow Cytometry.

BaF3 mouse pro-B cell line transfected with mouse CXCR6 and eGFP was stained with and either (A) Rat Anti-Mouse CXCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB2145P) or (B) Rat IgG2BPhycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR6 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CXCR6 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes treated with PMA and Calcium Ionomycin overnight were stained with Rat Anti-Mouse CXCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB2145P) and Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). Quadrant markers were set based on control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CXCR6 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: BaF3 mouse pro-B cell line transfected with mouse CXCR6 and eGFP and mouse splenocytes treated with PMA and Calcium Ionomycin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Alternate Names

Bonzo, CD186, CXCR6, STRL33

Entrez Gene IDs

10663 (Human); 80901 (Mouse); 100124593 (Rat)

Gene Symbol

CXCR6

UniProt

Additional CXCR6 Products

Product Documents for Mouse CXCR6 PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CXCR6 PE-conjugated Antibody

For research use only

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