Mouse DCAR/CLEC4B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2757
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 349214
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DCAR/CLEC4B
Gln42-Leu209
Accession # AAR13070
Gln42-Leu209
Accession # AAR13070
Specificity
Detects mouse DCAR/CLEC4B in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse DCAR/CLEC4B Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse bone marrow-derived dendritic cells
Sample: Mouse bone marrow-derived dendritic cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DCAR/CLEC4B
Dendritic Cell immuno-Activating Receptor alpha isoform (DCAR) is a type II membrane protein belonging to the C-type lectin domain family and is designated CLEC4B. Two isoforms of DCAR exist, a 209 amino acid (aa) residue alpha isoform and a 176 aa form with a 33 aa deletion at the membrane proximal region of the extracellular domain. The DCAR extracellular domain contains a carbohydrate-recognition domain (CRD) that shares 91% amino acid sequence identity with the CRD of DCIR/CLEC4A. The DCAR intracellular domain is very short and lacks the ITIM motif found in DCIR. DCAR and DCIR are considered paired immunoregulatory receptors where DCAR activates through the ITAM of its associated adaptor molecule FcR gamma.
Long Name
Dendritic Cell Immunoreceptor
Alternate Names
Aplra2, CLEC4B
Entrez Gene IDs
69810 (Mouse)
Gene Symbol
CLEC4B1
UniProt
Additional DCAR/CLEC4B Products
Product Documents for Mouse DCAR/CLEC4B Antibody
Product Specific Notices for Mouse DCAR/CLEC4B Antibody
For research use only
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