Mouse DCAR/CLEC4B Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2757

R&D Systems, part of Bio-Techne
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AF2757
AF2757-SP
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Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all DCAR/CLEC4B Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DCAR/CLEC4B
Gln42-Leu209
Accession # Q7TS58

Specificity

Detects mouse DCAR/CLEC4B in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse DCIR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse DCAR/CLEC4B Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse bone marrow-derived dendritic cells

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse DCAR/CLEC4B

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DCAR/CLEC4B

DCAR is a type II membrane protein belonging to the C-type lectin domain family and is designated CLEC4B. Two isoforms of DCAR, including the 209 amino acid (aa) residue alpha isoform and a 176 aa form with a 33 aa deletion at the membrane proximal region of the extracellular domain, exist. The DCAR extracellular domain contains a carbohydrate-recognition domain (CRD) that shares 91% amino acid sequence identity with the CRD of DCIR/CLEC4A. The DCAR intracellular domain is very short and lacks the ITIM motif found in DCIR. DCAR and DCIR are considered paired immunoregulatory receptors where DCAR activates through the ITAM of its associated adaptor molecule FcR gamma.

Long Name

Dendritic Cell Immunoreceptor

Alternate Names

Aplra2, CLEC4B

Entrez Gene IDs

69810 (Mouse)

Gene Symbol

CLEC4B1

UniProt

Q7TS58

Additional DCAR/CLEC4B Products

Product Documents for Mouse DCAR/CLEC4B Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse DCAR/CLEC4B Antibody

For research use only

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