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Mouse Dkk-1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM1765

R&D Systems, part of Bio-Techne
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BAM1765

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Monoclonal Rat IgG2B Clone # 259631

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dkk-1
Ser30-His272
Accession # O54908

Specificity

Detects mouse Dkk-1 in direct ELISAs and Western blots. Does not cross-react with recombinant human Dkk-1, recombinant mouse (rm) Dkk-2, rmDkk-3, or rmDkk-4.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse Dkk-1 Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5-15.5)

Western Blot

1 µg/mL
Sample: Recombinant Mouse Dkk-1 (Catalog # 1765-DK)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dkk-1

Dkk-1 is a Dickkopf-related protein that inhibits Wnt signaling by binding the Wnt co-receptor LRP5/6. It also binds Kremen-1 and Kremen-2. Dkk-1 is important in embryonic development.

Long Name

Dickkopf-1

Alternate Names

Dkk1

Entrez Gene IDs

22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)

Gene Symbol

DKK1

UniProt

Additional Dkk-1 Products

Product Documents for Mouse Dkk-1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Dkk-1 Biotinylated Antibody

For research use only

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