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Mouse EphB6 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF611

R&D Systems, part of Bio-Techne
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BAF611

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EphB6
Leu33-Ser587
Accession # O08644

Specificity

Detects mouse EphB6 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) EphA1, recombinant mouse (rm) EphA3, rmEphA4, rmEphrin-B1, rmEphA6, rmEphA7, recombinant rat EphA5, rmEphrin-B2, rmEphB1, rmEphB2, rmEphB3, rmEphB4, rrEphB1, rmEphA8, rmEphA2, and rhEphrin-B3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse EphB6 Biotinylated Antibody

Detection of Mouse EphB6 antibody by Western Blot.

Detection of Mouse EphB6 by Western Blot.

Western blot shows lysates of mouse thymus tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse EphB6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF611) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for EphB6 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse EphB6 Biotinylated Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse thymus tissue and mouse brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB6

EphB6, also known as Mep (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. However, it has been found that EphB6 contains substitutions within the kinase domain which results in EphB6 having no kinase activity (4). The ligands which bind EphB6 are unknown (2, 3). However, we have observed that the ephrin-B1 and ephrin-B2 ligands can bind the immobilized receptor in an ELISA-type assay. The extracellular domains of human and mouse EphB6 share 92% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
  4. Gurniak, C.B. and L.J. Berg (1996) Oncogene 13:777.

Long Name

Eph Receptor B6

Alternate Names

Hep, Mep

Entrez Gene IDs

2051 (Human); 13848 (Mouse)

Gene Symbol

EPHB6

UniProt

Additional EphB6 Products

Product Documents for Mouse EphB6 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse EphB6 Biotinylated Antibody

For research use only

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