Mouse Epimorphin/Syntaxin 2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2568
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 338930
Product Specifications
Immunogen
E. coli-derived recombinant mouse Epimorphin/Syntaxin 2
Met1-Arg189
Accession # Q00262
Met1-Arg189
Accession # Q00262
Specificity
Detects mouse Epimorphin/Syntaxin 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Epimorphin is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Epimorphin/Syntaxin 2 Antibody
Epimorphin/Syntaxin 2 in Embryonic Mouse Eye Cup.
Epimorphin/Syntaxin 2 was detected in immersion fixed frozen sections of embryonic mouse eye cup (13 d.p.c.) using 8 µg/mL Rat Anti-Mouse Epimorphin/Syntaxin 2 Monoclonal Antibody (Catalog # MAB2568) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Epimorphin/Syntaxin 2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse eye cup (13 d.p.c.)
Sample: Immersion fixed frozen sections of embryonic mouse eye cup (13 d.p.c.)
Western Blot
1 µg/mL
Sample: Recombinant Mouse Epimorphin/Syntaxin 2
Sample: Recombinant Mouse Epimorphin/Syntaxin 2
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Epimorphin/Syntaxin 2
Epimorphin, also known as syntaxin 2, is a member of the highly conserved syntaxin family. It is a type IV integral membrane protein with a C-terminal membrane anchor and an N-terminal functional domain. Epimorphin can assume membrane topology with the functional domain localized extracellularly or to the cytoplasmic face. Extracellular Epimorphin functions in morphogenesis while intracellular Epimorphin functions as a vesicle fusion protein. Mouse Epimorphin functional domain shares 97% and 91% amino acid sequence homology with rat and human Epimorphin, respectively.
Alternate Names
STX2, Syntaxin 2
Gene Symbol
STX2
UniProt
Additional Epimorphin/Syntaxin 2 Products
Product Documents for Mouse Epimorphin/Syntaxin 2 Antibody
Product Specific Notices for Mouse Epimorphin/Syntaxin 2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...