Mouse ESGP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4580
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse ESGP
Leu24-Lys84
Accession # Q2Q5T5
Leu24-Lys84
Accession # Q2Q5T5
Specificity
Detects mouse ESGP in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse ESGP Antibody
Detection of Mouse ESGP by Western Blot.
Western blot shows lysates of D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ESGP at approximately 9 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
ESGP in Mouse Testis.
ESGP was detected in perfusion fixed frozen sections of mouse testis using Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to late spermatids. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
ESGP in D3 Mouse Cell Line.
ESGP was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretion. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Mouse ESGP Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line
Sample: Immersion fixed D3 mouse embryonic stem cell line
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse testes
Sample: Perfusion fixed frozen sections of mouse testes
Western Blot
0.1 µg/mL
Sample: D3 mouse embryonic stem cell line
Sample: D3 mouse embryonic stem cell line
Reviewed Applications
Read 1 review rated 5 using AF4580 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ESGP
ESGP (ES cell and germ cell-specific protein) is a presumably secreted polypeptide that belongs to no known protein family. It is expressed specifically in pluripotential cell types, and does not appear to be involved in differentiation. Mouse ESGP precursor is 84 amino acids (aa) in length. It contains a 25 aa signal sequence with a 59 aa mature segment. There are no N-linked glycosylation sites. One potential 108 aa variant shows an alternate start site 24 aa upstream of the standard start site.
Long Name
Embryonic Stem Cell and Germ Cell Specific Protein
Alternate Names
EG653016
Entrez Gene IDs
653016 (Mouse)
Gene Symbol
MYMX
UniProt
Additional ESGP Products
Product Documents for Mouse ESGP Antibody
Product Specific Notices for Mouse ESGP Antibody
For research use only
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