Mouse FCRLB/FCRY Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4868

R&D Systems, part of Bio-Techne
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MAB4868
MAB4868-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 465905

View all FCRLB/FCRY Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FCRLB/FCRY
Ala18-Ser427
Accession # Q5DRQ8

Specificity

Detects mouse FCRLB/FCRY in direct ELISAs and Western blots. In Western blots, 75% cross-reactivity with recombinant human FCRLB is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse FCRLB/FCRY Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse FCRLB/FCRY (Catalog # 4868-FC)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRLB/FCRY

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 60 kDa protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified mouse IgG. Mouse FCRLB shares 82% and 94% aa sequence identity with human and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1‑3). The stalk region also contains di-Leu motifs and an unpaired cysteine (1‑4). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (4, 5). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (4, 5). It is upregulated in B cells by BAFF and LPS (3). Among non‑hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 4, 6). It is preferentially expressed in nonproliferating cells and at the onset of apoptosis (3, 5).

References

  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Masuda, K. et al. (2005) Gene 363:32.
  4. Chikaev, N.A. et al. (2005) Genomics 85:264.
  5. Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341.
  6. Inozume, T. et al. (2005) Int. J. Cancer 114:283.

Long Name

Fc Receptor-like B

Alternate Names

FcRL2, FCRLM2, FCRLY, FCRY, FREB-2

Entrez Gene IDs

127943 (Human); 435653 (Mouse); 498275 (Rat)

Gene Symbol

FCRLB

UniProt

Q5DRQ8

Additional FCRLB/FCRY Products

Product Documents for Mouse FCRLB/FCRY Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse FCRLB/FCRY Antibody

For research use only

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