Mouse FIZZ1/RELM alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1523
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Flow Cytometry, Fluorescence Microscopy, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 226033
Product Specifications
Immunogen
E. coli-derived recombinant mouse FIZZ1/RELM alpha
Asp24-Ser111
Accession # Q9EP95
Asp24-Ser111
Accession # Q9EP95
Specificity
Detects mouse FIZZ1/RELM alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) RELM beta or rmResistin is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse FIZZ1/RELM alpha Antibody
FIZZ1/RELM alpha in BV-2 Mouse Cell Line.
FIZZ1/RELMa was detected in immersion fixed BV-2 mouse microglia cell line using Rat Anti-Mouse FIZZ1/RELMa Monoclonal Antibody (Catalog # MAB1523) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (orange; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse FIZZ1/RELM alpha Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BV-2 mouse microglia cell line
Sample: Immersion fixed BV-2 mouse microglia cell line
Western Blot
1 µg/mL
Sample: Recombinant Mouse FIZZ1/RELM alpha
Sample: Recombinant Mouse FIZZ1/RELM alpha
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FIZZ1/RELM alpha
Long Name
Found in Inflammatory Zone 1/Resistin-like Molecule alpha
Alternate Names
RELM alpha, RETNLA, RETNLB
Gene Symbol
RETNLB
UniProt
Additional FIZZ1/RELM alpha Products
Product Documents for Mouse FIZZ1/RELM alpha Antibody
Product Specific Notices for Mouse FIZZ1/RELM alpha Antibody
For research use only
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