Mouse FOLR4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6124
Key Product Details
Validated by
Biological Validation
Species Reactivity
Mouse
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse FOLR4
Gly20-Ser100
Accession # Q9EQF4
Gly20-Ser100
Accession # Q9EQF4
Specificity
Detects mouse FOLR4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FOLR1, rhFOLR2, rhFOLR3, and rhFOLR4 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse FOLR4 Antibody
Detection of Mouse FOLR4 by Western Blot.
Western blot shows lysates of mouse CD+CD25+regulatory T cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse FOLR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6124) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FOLR4 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of FOLR4 in Mouse Splenocytes Gated on FoxP3 Cells by Flow Cytometry.
Mouse splenocytes gated on FoxP3 cells treated with 10 µL/mL anti-CD3/anti-CD28, Recombinant Mouse IL-2 (Catalog # 402-ML), and Human TGF-beta 1 (Catalog # 100-B) for 3 days to induce T regulatory cells (Tregs) were stained with Sheep Anti-Mouse FOLR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6124, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry FoxP3 Staining Buffer (Catalog # FC011).Applications for Mouse FOLR4 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse splenocytes gated on FoxP3 cells treated with anti-CD3/anti-CD28, Recombinant Mouse IL-2 (Catalog # 402-ML), and Human TGF-beta 1 (Catalog # 100-B) to induce T regulatory cells (Tregs) were fixed and permeabilized with Flow Cytometry FoxP3 Staining Buffer (Catalog # FC011)
Sample: Mouse splenocytes gated on FoxP3 cells treated with anti-CD3/anti-CD28, Recombinant Mouse IL-2 (Catalog # 402-ML), and Human TGF-beta 1 (Catalog # 100-B) to induce T regulatory cells (Tregs) were fixed and permeabilized with Flow Cytometry FoxP3 Staining Buffer (Catalog # FC011)
Western Blot
1 µg/mL
Sample: Mouse CD+CD25+ regulatory T cells
Sample: Mouse CD+CD25+ regulatory T cells
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FOLR4
FOLR4 (folate receptor 4; also FR4, FR‑ delta and FOLBP‑3) is a 35 kDa glycoprotein member of the folate receptor family. It is found on mouse Treg cells, particularly those classified as natural and CD4+ CD25- T cells that produce low amounts of IFN-gamma. Its function is unclear. Although folate family receptors can internalize folate, they are generally not the principal conduits for folate uptake. Mouse FOLR4 preproprecursor is 244 amino acids (aa) in length. It contains a 19 aa signal sequence plus a 225 aa proform. While there are no definitive structural motifs, there is presumably a C-terminal propeptide that gives rise to a GPI-linkage. There are three potential isoforms. One is 37 kDa in size and shows a 36 aa insertion after Glu155. Two others show a deletion of aa 47‑109, and a 17 aa substitution for aa 157‑244. Over aa 20‑100, mouse FOLR4 shares 84% and 70% aa identity with rat and human FOLR4, respectively.
Long Name
Folate Receptor 4
Alternate Names
FR-delta, LOC390243
Gene Symbol
IZUMO1R
UniProt
Additional FOLR4 Products
Product Documents for Mouse FOLR4 Antibody
Product Specific Notices for Mouse FOLR4 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...