Mouse FoxD3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5090

R&D Systems, part of Bio-Techne
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MAB5090
MAB5090-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 800602

View all FoxD3 Antibodies »

Product Summary for Mouse FoxD3 Antibody

Immunogen

E. coli-derived recombinant mouse FoxD3
Met1-Val130
Accession # Q61060

Specificity

Detects mouse FoxD3 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FoxD3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse FoxD3 Antibody

FoxD3 antibody in D3 Mouse Stem Cell Line by Immunocytochemistry (ICC).

FoxD3 in D3 Mouse Stem Cell Line.

FoxD3 was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse FoxD3 Monoclonal Antibody (Catalog # MAB5090) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse FoxD3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxD3

FoxD3 (forkhead box protein D3; also HNF3, HFH2 and Genesis) is a 50 kDa member of the winged helix transcription factor gene family.  It is expressed in the epiblast, migrating neural crest, and motoneurons plus interneuron progenitors of the developing spinal cord.  Mouse FoxD3 is 465 amino acids (aa) in length.  It contains a forkhead DNA binding domain (aa 131-208) plus an Ala-rich region (aa 248-464) that contains a Gly-rich motif (aa 369-399).  There appears to be one splice form that shows a four aa insert after Gly261.  Over aa 1-130, mouse FoxD3 shares 97% and 79% aa identity with rat and human FoxD3, respectively.   

Long Name

Forkhead Box D3

Alternate Names

CWH3, Genesis, HFH2

Entrez Gene IDs

27022 (Human); 15221 (Mouse)

Gene Symbol

FOXD3

UniProt

Q61060

Additional FoxD3 Products

Product Documents for Mouse FoxD3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse FoxD3 Antibody

For research use only

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