Mouse Galectin-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6667
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Galectin-2
Ser2-Glu130 (Gly36Val)
Accession # Q9CQW5
Ser2-Glu130 (Gly36Val)
Accession # Q9CQW5
Specificity
Detects mouse Galectin-2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human Galectin-2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Galectin-2 Antibody
Detection of Mouse Galectin‑2 by Western Blot.
Western blot shows lysates of mouse stomach tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Galectin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6667) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Galectin-2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Galectin‑2 in Mouse Intestine.
Galectin-2 was detected in perfusion fixed frozen sections of mouse intestine using Mouse Galectin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6667) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Galectin-2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine
Sample: Perfusion fixed frozen sections of mouse intestine
Western Blot
0.5 µg/mL
Sample: Mouse stomach tissue
Sample: Mouse stomach tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-2
Galectin-2 (Gal-2; also LEG2, HL14 and L-14-II) is a 14-15 kDa member of the S-type (1-CRD type) lectin family of proteins. It is a potentially secreted, noncovalent homodimer that is expressed by stomach mucosal columnar epithelium, small intestine Goblet cells and keratinocytes. Galectin-2 is reported to bind to CD29, and initiate CD8+ T cell apoptosis. Structurally, it serves to bind nonsialylated poly-NAcetyllactosamine motifs. Mouse Galectin-2 is 130 amino acids (aa) in length. There is one galectin domain (aa 4-130) that contains one dimerization interface (aa 123-128). Full-length mouse Galectin-2 shares 86% and 66% aa identity with rat and human Galectin-2, respectively.
Alternate Names
GAL2, Galectin2, LGALS2
Gene Symbol
LGALS2
UniProt
Additional Galectin-2 Products
Product Documents for Mouse Galectin-2 Antibody
Product Specific Notices for Mouse Galectin-2 Antibody
For research use only
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