Mouse GDF-7/BMP-12 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF779
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse GDF-7 (R&D Systems, Catalog # 779-G7)
Thr316-Arg461
Accession # P43029
Thr316-Arg461
Accession # P43029
Specificity
Detects mouse GDF-7 in direct ELISAs and Western blots. In these formats, approximately 10% cross‑reactivity with recombinant mouse (rm) GDF-5 and rmGDF-6 is observed and less than 1% cross-reactivity with rmGDF-1, rmGDF-3, rmGDF‑8, rmGDF-9, and recombinant human GDF-11 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse GDF-7/BMP-12 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse GDF-7/BMP-12 (Catalog # 779-G7)
Sample: Recombinant Mouse GDF-7/BMP-12 (Catalog # 779-G7)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GDF-7/BMP-12
Growth Differentiation Factor 7 (GDF-7), also known as bone morphogenetic protein (BMP) 12 or cartilage derived morphogenetic protein 3 (CDMP-3), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF-beta) superfamily. Mature GDF-7 contains 146 aa residues with the characteristic seven conserved cysteine residues. The bioactive GDF-7 is a homodimer with an apparent molecular weight of approximately 28 kDa. Mature GDF-5, GDF-6 and GDF-7, which share 80 - 86% identity with each other, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-7 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-7 are 98% identical. It has been reported that GDF-7 has multiple functions including regulation of chondrogenesis, bone morphogenesis, and neuron differentiation. GDF-7 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activiation of Smad proteins.
References
- Storm, E.E. et al. (1994) Nature 368:639.
- Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
- Francis-West, P.H. et al. (1999) Development 126:1035.
- Massague, J. et al. (2000) Genes and Dev. 14:627.
Long Name
Growth Differentiation Factor 7
Alternate Names
BMP-12, GDF7
Gene Symbol
GDF7
UniProt
Additional GDF-7/BMP-12 Products
Product Documents for Mouse GDF-7/BMP-12 Antibody
Product Specific Notices for Mouse GDF-7/BMP-12 Antibody
For research use only
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