Mouse Glutaredoxin 1/GLRX1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3119
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Glutaredoxin 1
Met1-Leu107
Accession # Q9QUH0
Met1-Leu107
Accession # Q9QUH0
Specificity
Detects endogenous human and mouse Glutaradoxin in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Glutaredoxin 1/GLRX1 Antibody
Detection of Mouse Glutaredoxin 1 by Western Blot.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Glutaredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Glutaredoxin 1 at approximately 12 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.Applications for Mouse Glutaredoxin 1/GLRX1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line
Sample: NIH-3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glutaredoxin 1/GLRX1
Glutaredoxin 1s or thioltransferases are a universally distributed family of small molecular weight proteins with thiol-disulfide exchange activity. Glutaredoxin 1 (GLRX1/GRX) is a 12 kDa protein with glutathione-disulfide oxidoreductase activity. Glutaredoxin 1s utilize the reducing power of glutathione to catalyze disulfide reductions in the presence of NADPH and glutathione reductase (the Glutaredoxin 1 system). The amino acid sequence of Glutaredoxin 1 in mouse is highly homologous with that in other mammals (81‑89%), and contains the conserved active sequence motif -Cys-Pro-Try-Cys-. Glutaredoxin 1 also catalyzes the formation of mixed disulfides (glutathionylation), which is an important redox regulatory mechanism, particularly in mammalian cells under oxidative stress conditions, to sense cellular redox potential.
Alternate Names
GLRX, Thioltransferase
Gene Symbol
GLRX
UniProt
Additional Glutaredoxin 1/GLRX1 Products
Product Documents for Mouse Glutaredoxin 1/GLRX1 Antibody
Product Specific Notices for Mouse Glutaredoxin 1/GLRX1 Antibody
For research use only
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