Mouse Glypican 6 Antibody

Detection of Mouse Glypican 6 by Western Blot

GDE2 substrates show impaired release in SOD1G93A mice. a Schematic of the sequential solubilization protocol used to separate hydrophilic non-membrane proteins (S1) from hydrophobic membrane proteins (S2). b Western blot demonstrating the successful enrichment of non-membrane protein (GAPDH) in the S1 fraction and membrane protein (Na/K ATPase) in the S2 fraction for WT and SOD1G93A transgenic mice. c Western blots visualizing the impaired production of non-membrane bound (S1) Glypican 6, Glypican 4, and Glypican 1 in the lumbar spinal cord of 4 month SOD1G93A mice. d Quantification of the reduced membrane release of Glypican 6, *p = 0.038; Glypican 4, *p = 0.016; and Glypican 1, *p = 0.030 relative to GAPDH. Graph represents mean ± SEM. Student’s t test, n = 4 WT, 4 SOD1G93A. Image collected and cropped by CiteAb from the following open publication (https://molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-017-0148-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Glypican 6 by Western Blot

GDE2 substrates show impaired release in SOD1G93A mice. a Schematic of the sequential solubilization protocol used to separate hydrophilic non-membrane proteins (S1) from hydrophobic membrane proteins (S2). b Western blot demonstrating the successful enrichment of non-membrane protein (GAPDH) in the S1 fraction and membrane protein (Na/K ATPase) in the S2 fraction for WT and SOD1G93A transgenic mice. c Western blots visualizing the impaired production of non-membrane bound (S1) Glypican 6, Glypican 4, and Glypican 1 in the lumbar spinal cord of 4 month SOD1G93A mice. d Quantification of the reduced membrane release of Glypican 6, *p = 0.038; Glypican 4, *p = 0.016; and Glypican 1, *p = 0.030 relative to GAPDH. Graph represents mean ± SEM. Student’s t test, n = 4 WT, 4 SOD1G93A Image collected and cropped by CiteAb from the following open publication (https://molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-017-0148-1), licensed under a CC-BY license. Not internally tested by R&D Systems.