Mouse HAI-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1107
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 170317
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse HAI-2B
aa 30-197 (del 37-93)
aa 30-197 (del 37-93)
Specificity
Detects rmHAI-2 in direct ELISAs and Western blots. Less than 5% cross-reactivity was observed with rmHAI-1 and rhHAI-2.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse HAI-2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse HAI-2
under non-reducing conditions only
Sample: Recombinant Mouse HAI-2
under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAI-2
Two alternatively spliced forms of HAI-2 are found in human tissues. HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain. Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trypsin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa.
Three alternatively spliced forms of HAI-2 have been found in mouse tissues. HAI-2A is a minor form expressed in mouse tissues. HAI-2B, with residues 37 through 93 spliced out and thus lack of the first Kunitz domain, is a major form in mouse tissues. HAI-2B is an effective inhibitor of both HGF activator and trypsin.
Long Name
HGF Activator Inhibitor Type 2
Alternate Names
HAI2
Gene Symbol
SPINT2
Additional HAI-2 Products
Product Documents for Mouse HAI-2 Antibody
Product Specific Notices for Mouse HAI-2 Antibody
For research use only
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