Mouse HB-EGF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8239
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Mouse
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rat IgG2A Clone # 917525R
Product Specifications
Immunogen
E. coli-derived recombinant mouse HB-EGF
Asp63-Leu148
Accession # Q06186
Asp63-Leu148
Accession # Q06186
Specificity
Detects mouse HB-EGF in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse HB-EGF Antibody
Mouse HB‑EGF ELISA Standard Curve.
Recombinant Mouse HB-EGF protein was serially diluted 2-fold and captured by Rat Anti-Mouse HB-EGF Monoclonal Antibody (Catalog # MAB8239) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse HB-EGF Monoclonal Antibody ( Catalog # MAB82391) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Mouse HB-EGF Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse HB‑EGF Monoclonal Antibody (Catalog # MAB82391).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse HB-EGF DuoSet ELISA Kit (Catalog # DY8239-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HB-EGF
Heparin-binding EGF-like growth factor, HB-EGF, is a 19-23 kDa glycoprotein, member of the EGF family of mitogens that exists in multiple forms as a result of heterogenous O-glycosylation. HB-EGF is a growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. It is required for normal cardiac valve formation and normal heart function and promotes smooth muscle cell proliferation. HB-EGF may be involved in macrophage-mediated cellular proliferation. The cDNA for mouse Proheparin-binding EGF-like growth factor encodes a 208 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB-EGF. Like EGF, TGF-alpha, and AR, HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF.
Long Name
Heparin Binding EGF-like Growth Factor
Alternate Names
Dtr, Dts, HBEGF, Hegfl
Gene Symbol
HBEGF
UniProt
Additional HB-EGF Products
Product Documents for Mouse HB-EGF Antibody
Product Specific Notices for Mouse HB-EGF Antibody
For research use only
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