Mouse ICOS Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB168
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Mouse
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 670306
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with mouse ICOS
Accession # Q9WVS0
Accession # Q9WVS0
Specificity
Detects mouse ICOS in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse ICOS Antibody
Detection of ICOS in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes treated with 1 ug/mL Concanavalin A for 3 days were stained with Rat Anti-Mouse ICOS Monoclonal Antibody (Catalog # MAB168, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse ICOS Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse splenocytes treated with Concanavalin A
Sample: Mouse splenocytes treated with Concanavalin A
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICOS
Mouse ICOS (inducible co-stimulator), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Mouse ICOS is a homodimeric type I transmembrane protein consisting of 200 amino acids (aa) with a putative 20 aa signal sequence, a 122 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% aa similarity with CD 28 and CTLA-4. Mouse and human ICOS share approximately 72% aa identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is upregulated within approximately 24‑48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
References
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
- Hutloff, A. et al. (1999) Nature 397:263.
- Aicher, A. et al. (2000) J. Immunol. 164:4689.
- Coyle, A.J. et al. (2000) Immunity 13:95.
- Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
Long Name
Inducible Co-stimulator
Alternate Names
AILIM, CD278, CRP-1
Gene Symbol
ICOS
UniProt
Additional ICOS Products
Product Documents for Mouse ICOS Antibody
Product Specific Notices for Mouse ICOS Antibody
For research use only
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