Mouse Iduronate 2-Sulfatase/IDS Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2486
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Iduronate 2‑Sulfatase/IDS
Thr36-Pro552
Accession # Q08890
Thr36-Pro552
Accession # Q08890
Specificity
Detects mouse Iduronate 2‑Sulfatase/IDS in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human IDS is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Iduronate 2-Sulfatase/IDS Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Iduronate 2-Sulfatase/IDS (Catalog # 2486-SU), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Iduronate 2-Sulfatase/IDS (Catalog # 2486-SU), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Iduronate 2-Sulfatase/IDS (Catalog # 2486-SU)
Sample: Recombinant Mouse Iduronate 2-Sulfatase/IDS (Catalog # 2486-SU)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Iduronate 2-Sulfatase/IDS
As a member of the sulfatase family, Iduronate 2-Sulfatase encoded by the IDS gene is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate (1, 2). It hydrolyzes the 2-sulfate group of the L-iduronate 2-sulfate units of the GAG. The IDS deficiency results in mucopolysaccharidosis II (MPS II or Hunter syndrome), an X-linked inborn error leading to lysosomal accumulation of the GAG and their excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe. The mouse IDS has sulfatase activity. In addition, recombinant human IDS is also available (Catalog # http://www.rndsystems.com/product_results.aspx?k=2449-SU">2449-SU).
References
- Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
- Neufeld, E.F. and J. Muenzer (2001) in The Metabolic and Molecular Basis of Inherited Disease, Scriver, C.R. et al. (eds.) p. 3421, New York, McGraw-Hill.
Alternate Names
SIDS
Gene Symbol
IDS
UniProt
Additional Iduronate 2-Sulfatase/IDS Products
Product Documents for Mouse Iduronate 2-Sulfatase/IDS Antibody
Product Specific Notices for Mouse Iduronate 2-Sulfatase/IDS Antibody
For research use only
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