Mouse IFN-alpha 4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10259

R&D Systems, part of Bio-Techne
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MAB10259-100
MAB10259-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 1020125

View all IFN-alpha 4 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived mouse IFN-alpha 4
Leu23-Glu186
Accession # P07351

Specificity

Detects mouse IFN-alpha 4 in direct ELISAs. In direct ELISAS, no cross-reactivity with recombinant mouse IFNA-1, -2, -6, -7, -9, -11, -12, -13, -15 or -16 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse IFN-alpha 4 Antibody

IFN-alpha 4 antibody in Mouse Spleen by Immunohistochemistry (IHC-P).

IFN-alpha 4 in Mouse Spleen.

IFN-alpha 4 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse IFN-alpha 4 Monoclonal Antibody (Catalog # MAB10259) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse IFN-alpha 4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse spleen using Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 4

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors (1). The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha-subunit) and IFNAR2 ( beta-subunit). This binding contributes to TNF-alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFN-alpha subtypes and the various IFN-alphas share about 80% sequence homology among them (4, 5). The mouse IFN-alpha 4 consists of 186 amino acids (aa) including a 24 aa signal peptide and a 162 aa IFN-alpha 4 mature domain. The mature mouse IFN-alpha 4 shares 58% and 76% sequence identity with mature human and rat IFN-alpha 4, respectively. The mouse IFN-alpha 4 reduces the local replication of murine cytomegalovirus in the tibia muscle (6). mIFN-A4 is a strong activator of Mx gene and  was shown to be very effective in reducing splenomegaly (7).

References

  1. Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
  2. Fung, K.Y. et al. (2013) Science 339:1088.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  5. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  6. Yeow, W.S. et al. (1998) J. Immunol. 15:2932.
  7. Gerlach, N. et al. (2009) European J. Immunol. 39:136.

Long Name

Interferon alpha 4

Alternate Names

IFNA4, IFNalpha 4

Entrez Gene IDs

3441 (Human); 15967 (Mouse)

Gene Symbol

IFNA4

UniProt

P07351

Additional IFN-alpha 4 Products

Product Documents for Mouse IFN-alpha 4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse IFN-alpha 4 Antibody

For research use only

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