Mouse IFN-alpha Antibody

One Neutralization Unit (NU) is the amount of antiserum required to neutralize one unit of mouse interferon alpha (Mu-IFN-alpha) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay using L929 cells as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758] with the exception that EMCV was used as the challenge virus. In this antiviral assay for interferon, about 5 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Mu-IFN-alpha provided by the National Institutes of Health [Ga02-901-511). This material is prepared specifically for effective neutralization of Mu-IFN-alpha.