Mouse IFN-beta Antibody
R&D Systems, part of Bio-Techne | Catalog # 32401-1
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Neutralization
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Recombinant mouse IFN-beta
Specificity
Detects mouse IFN-beta.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Applications for Mouse IFN-beta Antibody
Application
Recommended Usage
Neutralization
One neutralization unit is the amount of antiserum required to
neutralize one unit ofmouse interferon beta (Mu-IFN-beta) to a 50%
endpoint. Interferon was titrated with the use of the cytopathic effect
inhibition assay [Rubinstein, S., Familletti, P.C., and Pestka, S.
(1981) J. Virol. 37, 755-758] using L929 cells and
EMCV as the challenge virus. In this antiviral assay for interferon,
about 2.5 unit/ml of interferon is the quantity necessary to produce
acytopathic effect of 50%. The units are determined by use of a
laboratory standard calibrated with respect to the international
reference standard for Mu-IFN-beta provided by the National Institutes of
Health [Gb02-902-511]. This materialis prepared specifically for
effective neutralization of Mu-IFN-beta.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.3 M Sodium Bicarbonate, 0.2 M Sodium Chloride.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
Background: IFN-beta
References
Asselin-Paturel et al. (J Exp. Med, 2005) used 32400 in combination with Rabbit anti-mouse IFN-alpha (32100) to explore the role of Type I IFN in the migration and activation of dendritic cells. Kamath et al. (J. Immunol. 2005) used a similar cocktail to demonstrate that dendritic cell derived Type I IFN stimulates bystander T-cells.
Seimon et al. (PNAS, 2006) used 32400 to examine the role of IFN Beta in protecting LPS treated macrophages from apoptosis. Zheng et al. (J. Biol. Chem, 2006) used 32400 to examine the role of autocrine IFN Beta in RANKL stimulated iNOS expression in macrophages.
Seimon et al. (PNAS, 2006) used 32400 to examine the role of IFN Beta in protecting LPS treated macrophages from apoptosis. Zheng et al. (J. Biol. Chem, 2006) used 32400 to examine the role of autocrine IFN Beta in RANKL stimulated iNOS expression in macrophages.
Long Name
Interferon beta
Alternate Names
IFB, IFF, IFNB, IFNB1, IFNbeta
Gene Symbol
IFNB1
Additional IFN-beta Products
Product Documents for Mouse IFN-beta Antibody
Product Specific Notices for Mouse IFN-beta Antibody
For research use only
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