Mouse IFN-gamma R2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB773
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Rat
Applications
Validated:
CyTOF-ready, Flow Cytometry, Neutralization, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Hamster IgG Clone # MOB47
Product Specifications
Immunogen
Recombinant mouse IFN-gamma R2
Extracellular domain
Extracellular domain
Specificity
Detects mouse IFN‑ gamma R2 extracellular domain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IFN‑ gamma R2, recombinant mouse IFN-gamma R1, rhIFN-gamma R1, or rhIL-10 R beta is observed.
Clonality
Monoclonal
Host
Hamster
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse IFN-gamma R2 Antibody
IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma R2 Antibody.
Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse IFN-gamma R2 Monoclonal Antibody (Catalog # MAB773). The ND50 is typically 0.2-1 µg/mL.Applications for Mouse IFN-gamma R2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: This antibody has been shown to recognize mouse IFN-gamma R2 expressed on mouse T cells (Bach, E. et al., 1995, Science 270:1215). This application was not tested by R&D Systems.
Sample: This antibody has been shown to recognize mouse IFN-gamma R2 expressed on mouse T cells (Bach, E. et al., 1995, Science 270:1215). This application was not tested by R&D Systems.
Western Blot
1 µg/mL
Sample: Recombinant Mouse IFN-gamma R2
Sample: Recombinant Mouse IFN-gamma R2
Neutralization
Measured by its ability to neutralize IFN-gamma R2-mediated inhibition of EMCV-induced cytopathy in the L-929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Mouse IFN-gamma.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma R2
Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma. IFN‑ gamma R2 is required for signal transduction but does not bind IFN-gamma by itself.
Long Name
Interferon gamma Receptor 2
Alternate Names
AF-1, IFN-gR2, IFNgammaR2, IFNGR2, IFNGT1
Gene Symbol
IFNGR2
Additional IFN-gamma R2 Products
Product Documents for Mouse IFN-gamma R2 Antibody
Product Specific Notices for Mouse IFN-gamma R2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...