Mouse IFN-kappa Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5206
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse IFN-kappa
Leu22-Lys199 (Cys174Tyr)
Accession # Q7TSL0
Leu22-Lys199 (Cys174Tyr)
Accession # Q7TSL0
Specificity
Detects mouse IFN-kappa in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse IFN-kappa Antibody
Detection of Mouse IFN-kappa by Western Blot.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, A20 mouse B cell lymphoma cell line, and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse IFN-kappa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5206) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IFN-kappa at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Mouse IFN‑ kappa by Simple WesternTM.
Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and EL-4 mouse lymphoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for IFN-kappa at approximately 40 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse IFN-kappa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5206) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse IFN-kappa Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line and EL‑4 mouse lymphoblast cell line
Sample: RAW 264.7 mouse monocyte/macrophage cell line and EL‑4 mouse lymphoblast cell line
Western Blot
1 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line, A20 mouse B cell lymphoma cell line, and EL-4 mouse lymphoblast cell line
Sample: RAW 264.7 mouse monocyte/macrophage cell line, A20 mouse B cell lymphoma cell line, and EL-4 mouse lymphoblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-kappa
IFN-kappa (Interferon kappa) is a secreted, nonglycosylated polypeptide that belongs to the IFN-kappa subclass of the type I interferon family of cytokines. It is expressed by macrophages in mice, and monocytes, dendritic cells and keratinocytes in human. IFN-kappa induces TNF-alpha, IL-10 and MCP-1 production by monocytes. Mouse IFN-kappa precursor is 199 amino acids (aa) in length. It contains a 21 aa signal sequence, plus a 178 aa mature region. There are two intrachain disulfide bonds plus a fifth unpaired cysteine. Mature mouse IFN-kappa (aa 22‑199) shares 68% and 30% aa identity with rat and human IFN-kappa, respectively.
Long Name
Interferon kappa
Alternate Names
IFNK, IFNkappa
Gene Symbol
IFNK
UniProt
Additional IFN-kappa Products
Product Documents for Mouse IFN-kappa Antibody
Product Specific Notices for Mouse IFN-kappa Antibody
For research use only
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