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Mouse IGFBP-5 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF578

R&D Systems, part of Bio-Techne
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BAF578

Key Product Details

Species Reactivity

Mouse

Applications

ELISA Detection (Matched Antibody Pair), Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-5
Val15-Glu271
Accession # Q3UQV0

Specificity

Detects mouse IGFBP-5 in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant human (rh) IGFBP-5 is observed and 0.3% cross-reactivity with recombinant mouse (rm) IGF-I, rmIGF-II, rmIGFBP-I, rmIGFBP-2, rmIGFBP-3, and rmIGFBP-6 is observed. In Western blots, approximately 15% cross-reactivity with rhIGFBP-5 is observed and less than 1% cross-reactivity with rmIGFBP-1, rmIGFBP-2, rmIGFBP-3, and rmIGFBP-6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse IGFBP-5 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IGFBP-5 (Catalog # 578-B5)

Mouse IGFBP-5 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse IGFBP-5 Antibody (Catalog # AF578)
  • Standard: Recombinant Mouse IGFBP-5 Protein, CF (Catalog # 578-B5)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-5

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Mouse IGFBP-5 cDNA encodes a 271 amino acid (aa) residue precursor protein with a putative 19 aa residue signal peptide that is processed to generate the 252 aa residue mature protein. Mouse, human and rat IGFBP-5 share 97% identity. IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. IGFBP-5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracelluar matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21 kDa fragment.

References

  1. James, P.L. et al. (1993) J. Biol. Chem. 268:22305.
  2. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  3. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.

Long Name

Insulin-like Growth Factor Binding Protein 5

Alternate Names

IGFBP5

Entrez Gene IDs

3488 (Human); 16011 (Mouse)

Gene Symbol

IGFBP5

UniProt

Additional IGFBP-5 Products

Product Documents for Mouse IGFBP-5 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IGFBP-5 Biotinylated Antibody

For research use only

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