Mouse IL-22 Antibody

IL‑10 Secretion Induced by IL‑22 and Neutralization by Mouse IL‑22 Antibody.

Recombinant Mouse IL-22 (Catalog # 582-ML) stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Mouse IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582). The ND₅₀ is typically 0.5-2.5 µg/mL.

Detection of Mouse Mouse IL-22 Antibody by Flow Cytometry

Imiquimod increases IL-17 and IL-22 in both gamma delta and alpha beta T cells in skin.Skin and ear of wild type C57BL/6 mice were treated by topical application of imiquimod cream (Fougera, n=4) or control cream (Vaseline, n=3) for 6 consecutive days. (a) Weight loss of imiquimod and control cream treated mice monitored daily. (b) Photographs of imiquimod and control cream treated skin and ear; photos were taken at day 6. H&E stained ear and back skin sections of imiquimod treated and control mice. Scale bar, 200 μm. (c) Thickness of skin measured by Digimatic Caliper at day 6 in control and imiquimod treated mice. Data showed represents average of at least two measurements. (d) Ear thickness of imiquimod and control cream treated mice monitored daily. Ear thickness was measured using Digimatic Caliper. (e) Quantitative PCR analysis of Th17 associated cytokines and Foxp3 in skin after 6 days of imiquimod and control cream treatment. (f) Representative flow cytometric analysis of TCR gamma delta+V gamma4+IL-17+ (upper raw) and TCR gamma delta+V gamma4+IL-22+ cells (lower row) in the skin of control (n=3) and imiquimod treated mice (n=4). (g) Representative flow cytometric analysis of single-positive TCR beta+IL-17+ (upper raw) or TCR beta+IL-22+ (lower raw) cells in the skin of control (n=3) and imiquimod treated mice (n=4). Data representative of more than three experiments, results are shown as mean±s.e.m., significance determined by unpaired two-tailed Student's t-test (*P<0.05; **P<0.01; ***P<0.001). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26416167), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Mouse IL-22 Antibody by Flow Cytometry

Imiquimod increases IL-17 and IL-22 in both gamma delta and alpha beta T cells in skin.Skin and ear of wild type C57BL/6 mice were treated by topical application of imiquimod cream (Fougera, n=4) or control cream (Vaseline, n=3) for 6 consecutive days. (a) Weight loss of imiquimod and control cream treated mice monitored daily. (b) Photographs of imiquimod and control cream treated skin and ear; photos were taken at day 6. H&E stained ear and back skin sections of imiquimod treated and control mice. Scale bar, 200 μm. (c) Thickness of skin measured by Digimatic Caliper at day 6 in control and imiquimod treated mice. Data showed represents average of at least two measurements. (d) Ear thickness of imiquimod and control cream treated mice monitored daily. Ear thickness was measured using Digimatic Caliper. (e) Quantitative PCR analysis of Th17 associated cytokines and Foxp3 in skin after 6 days of imiquimod and control cream treatment. (f) Representative flow cytometric analysis of TCR gamma delta+V gamma4+IL-17+ (upper raw) and TCR gamma delta+V gamma4+IL-22+ cells (lower row) in the skin of control (n=3) and imiquimod treated mice (n=4). (g) Representative flow cytometric analysis of single-positive TCR beta+IL-17+ (upper raw) or TCR beta+IL-22+ (lower raw) cells in the skin of control (n=3) and imiquimod treated mice (n=4). Data representative of more than three experiments, results are shown as mean±s.e.m., significance determined by unpaired two-tailed Student's t-test (*P<0.05; **P<0.01; ***P<0.001). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26416167), licensed under a CC-BY license. Not internally tested by R&D Systems.