Mouse IL-9 Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC409R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Intracellular Staining by Flow Cytometry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 222604
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-9
Gln19-Pro144
Accession # P15247.1
Gln19-Pro144
Accession # P15247.1
Specificity
Detects mouse IL-9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human IL-9 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse IL-9 Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Sample: Mouse splenocytes treated with anti-CD3/anti-CD28, Recombinant Mouse IL-4 (Catalog # 404-ML), Human TGF-beta 1 (Catalog # 100-B), and PMA/Ionomycin to induce Th9 development.
Sample: Mouse splenocytes treated with anti-CD3/anti-CD28, Recombinant Mouse IL-4 (Catalog # 404-ML), Human TGF-beta 1 (Catalog # 100-B), and PMA/Ionomycin to induce Th9 development.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-9
Mouse IL-9 was originally identified as a T cell-derived T cell growth factor III/P40 which could support the long term growth of certain mouse T helper clones in the absence of antigen or antigen-presenting cells. IL-9 can also prolong the in vitro survival of other T cell clones as well as potentiate the IL-2 dependent proliferation of mouse fetal thymocytes. However, this cytokine has no growth-stimulating activity on mouse cytolytic T cell clones or fresh T cells. Mouse IL-9 also has mast cell enhancing activity (MEA) and can enhance the mIL-3- or mIL-4-dependent proliferation of mouse bone marrow-derived mast cells. Furthermore, IL-9 will synergize with erythropoietin to support erythroid colony formation in vitro. The mouse IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a predicted molecular mass of 14 kDa. Mouse IL-9 contains four potential N-linked glycosylation sites and the native mIL-9 is a highly glycosylated protein. Human and mouse IL-9 share 56% amino acid sequence homology. Although mouse IL-9 is active on human cells, human IL-9 is not active on mouse cells.
References
- Renauld, J.E. et al. (1995) J. Leukoc. Biol 57:303.
Long Name
Interleukin 9
Alternate Names
IL9
Gene Symbol
IL9
UniProt
Additional IL-9 Products
Product Documents for Mouse IL-9 Alexa Fluor® 647-conjugated Antibody
Product Specific Notices for Mouse IL-9 Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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