Mouse Integrin  alpha10 Alexa Fluor® 350-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB9757U

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 885501

View all Integrin alpha 10 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha10 beta1
Phe23-Thr1119 (Integrin alpha10) and Gln21-Asp728 (Integrin beta1)
Accession # NP_001289400.1 (Integrin alpha10) and P09055 (Integrin beta1)

Specificity

Detects mouse Integrin alpha10 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Intagrin alpha2 beta1 was observered.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 10

Integrin alpha10 beta1 is one of twelve integrin family adhesion receptors that share the beta1 subunit (1‑3). The non‑covalent heterodimer of 160 kDa alpha11 and 130 kDa beta1/CD29 type I transmembrane glycoprotein subunits is expressed mainly on chondrocytes within cartilage, but also in fibrous connective tissues such as heart valves and ligaments (3, 4). The alpha10 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3, 5). The beta1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside‑out) signaling convert it to its most active, extended and open conformation (1, 2). The 1100 amino acid (aa) mouse  alpha10 extracellular domain (ECD) shares 96% aa sequence identity with  rat and 88‑89% with human, rabbit, porcine, canine and bovine alpha10, while the 708 aa mouse beta1 ECD shares 98% aa identity with rat and 93‑94% with human, bovine, porcine, ovine, canine and feline beta1. A potential mouse alpha10 splice variant diverges at aa 1039 and is terminated prematurely. If translated, this variant would result in a secreted protein (6). I domain‑containing beta1 integrins alpha1 beta1, alpha2 beta1, alpha10 beta1 and alpha11 beta1 all bind collagens; all but alpha11 beta1 also bind laminins (5, 7, 8). During cartilage differentiation, alpha10 beta1 is thought to be the main integrin binding type II and IX cartilage collagens (3‑5, 7‑10). However, deletion of mouse alpha10 causes a mild phenotype including slightly shortened bones and narrowed hypertrophic zones, indicating that another collagen‑binding integrin, likely alpha2 beta1, may compensate for alpha10 beta1 functions (11). Migration of melanoma cells has been noted to correlate with alpha10 beta1 expression (12).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Camper, L. et al. (1998) J. Biol. Chem. 273:20383.
  4. Camper, L. et al. (2001) Cell Tiss. Res. 306:107.
  5. Tulla, M. et al. (2001) J. Biol. Chem. 276:48206.
  6. Bengtsson, T. et al. (2001) Matrix Biol. 20:565.
  7. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
  8. Popova, S.N. et al. (2007) Acta Physiol. 190:179.
  9. Varas, L. et al. (2007) Stem Cells Dev. 16:965.
  10. Gigout, A. et al. (2008) J. Biol. Chem. 283:31522.
  11. Bengtsson, T. et al. (2004) J. Cell Sci. 118:939.
  12. Wenke, A.K. et al. (2007) Cell Oncol. 29:373.

Alternate Names

ITGA10, PRO827

Entrez Gene IDs

8515 (Human); 213119 (Mouse); 310683 (Rat)

Gene Symbol

ITGA10

UniProt

NP_001289400.1 (Integrin alpha10) and P09055 (Integrin beta1)

Additional Integrin alpha 10 Products

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Certificate of Analysis

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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