Mouse Integrin alpha2/CD49b Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1740
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha2/CD49b
Tyr27-Thr1129
Accession # Q62469
Tyr27-Thr1129
Accession # Q62469
Specificity
Detects mouse Integrin alpha2/CD49b in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately10% cross‑reactivity with recombinant human Integrin alphaM is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha5 and rmIntegrin alphaE is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Integrin alpha2/CD49b Antibody
Detection of Integrin alpha2/CD49b in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 PE-conjugated Monoclonal Antibody (Catalog # FAB8319P) and either (A) Sheep Anti-Mouse Integrin a2/CD49b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1740) or (B) Normal Sheep IgG Control (Catalog # 5-001-A) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).
Integrin alpha2/CD49b in Mouse Splenocytes.
Integrin a2/CD49b was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Integrin a2/CD49b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1740) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse Integrin alpha2/CD49b Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Integrin alpha2/CD49b
Sample: Recombinant Mouse Integrin alpha2/CD49b
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 2/CD49b
Alternate Names
CD49b, ITGA2, VLA-2 alpha
Gene Symbol
ITGA2
UniProt
Additional Integrin alpha 2/CD49b Products
Product Documents for Mouse Integrin alpha2/CD49b Antibody
Product Specific Notices for Mouse Integrin alpha2/CD49b Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...