Mouse Integrin  alpha5/CD49e Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1754

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot

Cited:

Immunocytochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 235112

View all Integrin alpha 5/CD49e Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha5/CD49e
Phe45-Asn999 (Gln68Glu)
Accession # P11688

Specificity

Detects mouse Integrin alpha5/CD49e in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse Integrin  alpha5/CD49e Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: B16-F1 mouse melanoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed L-929 mouse fibroblast cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse Integrin  alpha5/CD49e
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5/CD49e

Integrin alpha5 subunit, also called CD49e, associates with the Integrin beta1 subunit (CD29) to form the VLA-5 complex. It is expressed on thymocytes, mast cells, activated T lymphocytes, and some mouse cell lines. It functions as a receptor for fibronectin (1‑4).

References

  1. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
  2. Kinashi, T. and T.A. Springer (1994) Blood Cells 20:25.
  3. Halvorson, M.J. et al. (1995) J. Immunol. 55:4567.
  4. Uhlenkott, E.C. et al. (1996) Clin. Exp. Metastasis 14:125.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Entrez Gene IDs

3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)

Gene Symbol

ITGA5

UniProt

P11688

Additional Integrin alpha 5/CD49e Products

Product Documents for Mouse Integrin  alpha5/CD49e Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Integrin  alpha5/CD49e Antibody

For research use only

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