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Mouse Integrin  alpha9 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3827

R&D Systems, part of Bio-Techne
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AF3827
AF3827-SP

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot

Cited:

Flow Cytometry, IHC/IF, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integin alpha9
Try31-Val979
Accession # NP_598482

Specificity

Detects mouse Integrin alpha9 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human Integrin alpha9 is observed and less than 5% cross-reactivity with recombinant mouse Integrin alpha3 and alpha4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Integrin  alpha9 Antibody

Integrin alpha 9 antibody in Mouse Liver Tissue by Immunohistochemistry (IHC-Fr).

Integrin alpha 9 in Mouse Liver Tissue.

Integrin a9 was detected in perfusion fixed frozen sections of mouse liver tissue using Goat Anti-Mouse Integrin a9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3827) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human Integrin alpha 9 by Immunocytochemistry/Immunofluorescence

Detection of Human Integrin alpha 9 by Immunocytochemistry/Immunofluorescence

Morphology and protein expression of hSKPs. (a) SEM micrographs of nanofibrous scaffolds showing morphology of hSKPs at 7 days of culture. (b) Immunofluorescent images of hSKPs grown on nanofibrous scaffolds showing expression of integrin alpha-9 (green), fibronectin (red) and procollagen I & III (green). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28860484), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Integrin alpha 9 by Immunocytochemistry/Immunofluorescence

Detection of Mouse Integrin alpha 9 by Immunocytochemistry/Immunofluorescence

Intergins alpha4/ alpha9 are responsible for the action of osteopontin (OPN) in the augmentation of macrophage (Mφ) M2 polarization and angiogenic capacity. (A) Relative mean fluorescence intensities (MFIs) of integrins alpha4/ alpha9 were measured by FACS analysis in each Mφ subset. Anti-integrin alpha4 or alpha9 antibodies (Abs) and each isotype-matched control Ab were used at 10 µg/mL, n = 4 [***p < 0.001 vs. untreated, ###p < 0.001 vs. interleukin (IL)-10 alone]. (B) Representative confocal laser scanning immunofluorescence overlay images of integrin alpha4 (green) and DAPI (blue), as well as those of integrin alpha9 (red) and DAPI (blue) in Mφ (–) and Mφ (IL-10 + IL-18). Scale bar represents 20 µm. (C) Relative MFI of CD163 was measured by FACS analysis in each Mφ subset. Anti-integrin alpha4 or alpha9 Abs and each isotype-matched control Ab were used at 10 µg/mL, n = 4 (***p < 0.001 vs. untreated, ###p < 0.001 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). (D) The total areas and lengths of tube-like structures were determined by the Matrigel tube formation assay where b.End5 was cocultured with each Mφ subset. Anti-integrin alpha4 or alpha9 Abs and each isotype-matched control Ab were used at 10 µg/mL, n = 6 (***p < 0.001, **p < 0.01, *p < 0.05 vs. untreated, ###p < 0.001, ##p < 0.01, #p < 0.05 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). Integrin alpha4 = ITGA4; Integrin alpha9 = ITGA9. All data are expressed as means ± SEM and were analyzed by a one-way ANOVA followed by Tukey’s test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29559970), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Integrin  alpha9 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: D3 mouse embryonic stem cell line

Immunohistochemistry

1-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Integrin  alpha9

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 9

Integrin alpha9 is a 150 kDa type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta1 integrin. alpha9 beta1 binds to VEGF-C, VEGF-D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse integrin alpha9 contains three beta-propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha9 ECD is absolutely conserved in mouse alpha9 ECD (Arg566-Val567). The ECD of mouse integrin alpha9 shares 95% and 89% aa sequence identity to rat and human integrin alpha9, respectively.

Alternate Names

ITGA4L, ITGA9, RLC-alpha

Entrez Gene IDs

3680 (Human); 104099 (Mouse); 685004 (Rat)

Gene Symbol

ITGA9

UniProt

Additional Integrin alpha 9 Products

Product Documents for Mouse Integrin  alpha9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Integrin  alpha9 Antibody

For research use only

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