Mouse IRF3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4454
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse IRF3
Thr201-Ile419
Accession # P70671
Thr201-Ile419
Accession # P70671
Specificity
Detects mouse IRF3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse IRF3 Antibody
Detection of Mouse IRF3 by Western Blot.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse IRF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4454) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IRF3 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse IRF3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line
Sample: NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRF3
IRF3 is a member of the interferon regulator transcription factor family. IRF3 is typically found inactive in the cytoplasm. IRF3 is activated by the toll like receptors (TLR3 and TLR4), as well as, viral entry. Upon activation and phosphorylation, IRF3 heterodimerizes with CREBBP, which translocates to the nucleus to stimulate transcription. IRF3 does not contain transactivation function on its own; it must either recruit or be part of a complex of transcription factors to stimulate gene transcription.
Long Name
Interferon Regulatory Factor 3
Alternate Names
interferon regulatory factor 3, IRF-3
Gene Symbol
IRF3
UniProt
Additional IRF3 Products
Product Documents for Mouse IRF3 Antibody
Product Specific Notices for Mouse IRF3 Antibody
For research use only
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