A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse IRF5 PE-conjugated Monoclonal Antibody (Catalog # IC8447P, filled histogram) or isotype control antibody (IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Detection of IRF5 in A20 cells by Flow Cytometry

A20 cells were stained with Rat Anti-Mouse IRF5 PE-conjugated Monoclonal Antibody (Catalog # IC8447P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Mouse IRF5 PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/10⁶ cells
Sample: A20 mouse B cell lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005); A20 mouse B cell lymphoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer kit (FC012)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IRF5

IRF5 is a member of the IRF family of transcription factors, a family characterized by a helix-turn-helix DNA binding domain enriched in tryptophan repeats. IRF family members show diverse cellular regulation of interferon-stimulated gene transcription, viral-mediated gene activation, apoptosis, differentiation, and cellular growth. IRF5, along with IRF7, are the key mediators of TLR signaling. IRF5 forms heterodimers with IRF3 both of which are necessary for interferon gene transcription. IRF5 knock out mice indicate that IRF5 is critical for induction of apoptosis.

Long Name

Interferon Regulatory Factor 5

Alternate Names

IBD14, interferon regulatory factor 5, IRF-5, SLEB10

Entrez Gene IDs

3663 (Human); 27056 (Mouse); 296953 (Rat)

Gene Symbol

IRF5

UniProt

P56477

Additional IRF5 Products

Product Documents for Mouse IRF5 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse IRF5 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse IRF5 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC8447P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all IRF5 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse IRF5 PE-conjugated Antibody

Detection of IRF5 in A20 Mouse Cell Line by Flow Cytometry.