Mouse ISLR-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4650
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ISLR-2
Cys20-Leu590
Accession # Q5RKR3
Cys20-Leu590
Accession # Q5RKR3
Specificity
Detects mouse ISLR-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human ISLR-2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse ISLR-2 Antibody
Detection of Mouse ISLR-2 by Western Blot.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ISLR-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4650) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ISLR-2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
ISLR‑2 in Embryonic Mouse Brainstem.
ISLR-2 was detected in immersion fixed frozen sections of embryonic mouse brainstem (13 d.p.c.) using Sheep Anti-Mouse ISLR-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4650) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse ISLR-2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse embryonic retina cells (E15)
Sample: Immersion fixed mouse embryonic retina cells (E15)
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse brainstem (13 d.p.c.)
Sample: Immersion fixed frozen sections of embryonic mouse brainstem (13 d.p.c.)
Western Blot
1 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line and EL-4 mouse lymphoblast cell line
Sample: NIH-3T3 mouse embryonic fibroblast cell line and EL-4 mouse lymphoblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ISLR-2
ISLR2 (Ig‑superfamily containing LRR protein 2; also SST273) is a 100‑110 kDa member of the LRR family of proteins. It is expressed on developing motor, sensory and interneurons. Mouse ISLR2 is 745 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 571 aa extracellular domain (ECD) and a 132 aa cytoplasmic region. The ECD shows multiple LRRs (aa 21‑231) and one Ig‑like domain (aa 251‑374). Mouse ISLR2 apparently has one alternate start site at Met130. Over aa 20‑590, mouse ISLR2 is 89% and 98% aa identical to human and rat ISLR2, respectively.
Long Name
Immunoglobulin Superfamily Containing Leucine-rich Repeat 2
Alternate Names
ISLR2
Gene Symbol
ISLR2
UniProt
Additional ISLR-2 Products
Product Documents for Mouse ISLR-2 Antibody
Product Specific Notices for Mouse ISLR-2 Antibody
For research use only
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