Mouse Jagged 2 Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4748T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 746513
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Jagged 2
Met27-Leu1084
Accession # Q9QYE5
Met27-Leu1084
Accession # Q9QYE5
Specificity
Detects mouse Jagged 2 in direct ELISAs. In direct ELISAs, no cross-reactivity
with recombinant human (rh) Jagged 1, rhJagged 2, recombinant rat Jagged 1 is
observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Jagged 2 Alexa Fluor® 594-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: D3 mouse embryonic stem cell line
Sample: D3 mouse embryonic stem cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Jagged 2
Mouse Jagged 2 is a 150 kDa (1) member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins, and thereby regulate cell fate determinations during development (2-6). It is a type 1 transmembrane protein that is synthesized as a 1247 amino acid (aa) precursor. It contains a 23 aa signal sequence, a 1059 aa extracellular region, a 21 aa transmembrane region, and a 144 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats, many of which are also sites of calcium binding, and a cysteine-rich region just proximal to the transmembrane segment (3). Mouse Jagged 2 shares 93% and 90% aa sequence identity with rat and human Jagged 2, respectively. Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (3). In 2-week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (3). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (3). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, gamma delta T cell lineage differentiation, and the establishment of functional natural killer cell lines (4 - 6).
References
- Tsai, S. et al. (2000) Blood, 96:950.
- Shawber, C. et al. (1996) Dev. Biol. 180:370.
- Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
- Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
- DeHart, S. et al. (2005) Blood 105:3521.
- de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.
Alternate Names
HJ2, JAG2
Gene Symbol
JAG2
UniProt
Additional Jagged 2 Products
Product Documents for Mouse Jagged 2 Alexa Fluor® 594-conjugated Antibody
Product Specific Notices for Mouse Jagged 2 Alexa Fluor® 594-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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