Mouse JAM-4/IGSF5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3836
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse JAM‑4/IGSF5
Leu15-Ala139
Accession # NP_082354
Leu15-Ala139
Accession # NP_082354
Specificity
Detects mouse JAM‑4/IGSF5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) JAM-A, rmJAM-B, and rmJAM-C is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse JAM-4/IGSF5 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse JAM-4/IGSF5
Sample: Recombinant Mouse JAM-4/IGSF5
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: JAM-4/IGSF5
JAM-4, also known as IGSF5, is a 93 kDa member of the CTX (Cortical Thymocyte Marker in Xenopus) subfamily of the Ig superfamily. It is a type I transmembrane protein with an N-terminal extracellular V-type and C2 type Ig domain separated by a J segment, a transmembrane domain and a cytoplasmic tail with a PDZ binding motif. JAM-4 is expressed in intestinal, renal, and mammary epithelial cells, spermatogonia, and hematopoietic stem cells. It is a cell adhesion molecule that mediates homophilic interaction. JAM-4 also interacts with the intracellular membrane-associated guanylate kinase inverted 1 (MAGI-1). An additional isoform containing a 100 residue insertion between Gln 125 and Asp 126 has been reported. Over the region used as immunogen, mouse JAM-4 shares 82% and 63% amino acid sequence identity with rat and human JAM-4, respectively.
Long Name
Junctional Adhesion Molecule 4
Alternate Names
IGSF5, JAM4, JCAM
Gene Symbol
IGSF5
UniProt
Additional JAM-4/IGSF5 Products
Product Documents for Mouse JAM-4/IGSF5 Antibody
Product Specific Notices for Mouse JAM-4/IGSF5 Antibody
For research use only
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