Mouse Klotho Antibody

Detection of Human Klotho by Western Blot

Ectopic expression of Klotho protects renal mitochondrial homeostasis and inhibits tubular senescence in an accelerated aging mouse model. (a) Renal expression of PGC‐1 alpha mRNA in different groups was assessed by real‐time PCR. *p < .05 versus control mice; †p < .05 versus d‐gal‐treated mice (n = 5–6). (b) Representative micrographs show renal expression of PGC‐1 alpha. Paraffin sections were immunostained for PGC‐1 alpha. Arrows indicate positive staining. Scale bar, 50 µm. (c–h) Representative (c) Western blots and graphical representations of (d) phospho‐PGC‐1 alpha, (e) TFAM, (f) COX1, (g) Cytb, and (h) TOMM20 protein expression levels in different groups are presented. *p < .05 versus control mice; †p < .05 versus d‐gal‐treated mice (n = 5–6). (i) Quantification of renal mitochondrial mass is shown. Mitochondrial mass was determined by the fluorescence intensity of MitoTracker deep red staining normalized to DAPI. *p < .05 versus control mice; †p < .05 versus d‐gal‐treated mice (n = 5–6). (j) Representative micrographs show mitochondrial ROS production and ultrastructure. They were assessed by mitoSOX staining and electron microscopy analyses, respectively. The administration of d‐gal induced mitochondrial ROS production, mitochondrial swelling, and cristae disorganization in renal tubular cells. Arrows indicate positive staining. Ectopic expression of Klotho inhibited mitochondrial ROS production and preserved normal structure of mitochondria. Scale bar in mitoSOX staining, 50 μm. Scale bar in TEM, 1 μm. TEM, transmission electron microscopy. (k) Representative staining micrographs show renal expression of p16INK4A, SA‐ beta‐gal activity, and gammaH2AX. Paraffin kidney sections were stained with antibodies against p16INK4A and gammaH2AX. Frozen kidney sections were stained for SA‐ beta‐gal activity. Arrows indicate positive staining. Scale bar, 25 μm. (l–n) Representative (l) Western blots and graphical representations of (m) p16INK4A and (n) p19ARF protein expression levels in kidneys are presented. *p < .05 versus control mice; †p < .05 versus d‐gal‐treated mice (n = 5–6) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31318148), licensed under a CC-BY license. Not internally tested by R&D Systems.