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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 95218

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse L‑Selectin/CD62L
Met1-Asn332
Accession # P18337

Specificity

Detects mouse L‑Selectin/CD62L in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human L-Selectin, recombinant mouse (rm) E-Selectin, or rmP-Selectin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse L-Selectin/CD62L Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: L-Selectin/CD62L

L‑Selectin (Leukocyte Selectin; also known as CD62L, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL‑14 antigen, DREG, and lymph node homing receptor), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L‑Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is
90‑100 kDa. Human and mouse L‑Selectin share 76% amino acid sequence homology. L‑Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L‑Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium. This initial interaction involving ICAM-1 and VCAM-1 leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble L‑Selectin are present in the biological fluids of apparently normal individuals. Levels of L‑Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Alternate Names

CD62L, hLHRc, LAM1, LECAM1, Leu-8, LNHR, LSEL, Lyam-1, LYAM1, PLNHR, SELL, TQ1

Entrez Gene IDs

6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)

Gene Symbol

SELL

UniProt

Additional L-Selectin/CD62L Products

Product Documents for Mouse L-Selectin/CD62L Alexa Fluor® 647-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse L-Selectin/CD62L Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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