Can this LAG-3 antibody be used in ELISA?

We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).

I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?

There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.

Hello, I want to do IHC with LAG3 antibody, but I don’t know how to choose the positive control tissue or cell line. Can you help?

LAG-3 protein is expressed in activated NK and T-lymphocytes. As such, finding a good tissue or cell line for positive control testing may be difficult. Some of the papers linked below may offer some help: https://pathsocjournals.onlinelibrary.wiley.com/doi/abs/10.1002/path.1679 http://clincancerres.aacrjournals.org/content/21/17/3969.full.pdf https://www.researchgate.net/profile/Devinder_Gill2/publication/7024802_Expression_of_LAG-3_by_tumor-infiltrating_lymphocytes_is_coincident_with_the_suppression_of_latent_membrane_antigen-specific_CD8_T-cell_function_in_Hodgkin_lymphoma_patients/links/0fcfd50bfccc52342f000000.pdf http://cancerres.aacrjournals.org/content/75/15_Supplement/271.short For some data for normal tissue and cell line expression of the LAG-3 protein, you can check the Human Protein Atlas site. For tissues, appendix, lymph node, and tonsil tissue seem to show the best level of expression for this protein: http://www.proteinatlas.org/ENSG00000089692-LAG3/tissue. With regard to cell lines, the HDLM-2 cell line by far has the best level of expression: http://www.proteinatlas.org/ENSG00000089692-LAG3/cell/HPA013967. I hope this data will help you to select a good control for your experiment. Please let us know if you have any additional questions.

Mouse LAG-3 PE-conjugated Antibody

Name: Mouse LAG-3 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB33281P

R&D Systems, part of Bio-Techne | Catalog # FAB33281P

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MAB33281

Mouse LAG-3 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB33281P

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Detection of LAG-3 in Mouse Splenocytes by Flow Cytometry.

Detection of LAG-3 in CTLL-2 mouse cytotoxic T cell line by Flow Cytometry

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