Mouse Leptin R Antibody
R&D Systems, part of Bio-Techne | Catalog # AF497
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Leptin R
Ala20-Gly839
Accession # Q3US58
Ala20-Gly839
Accession # Q3US58
Specificity
Detects mouse Leptin R in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Leptin R is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Leptin R Antibody
Leptin R in Rat Brain.
Leptin R was detected in perfusion fixed frozen sections of rat brain using 15 µg/mL Goat Anti-Mouse Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF497) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in the choroid plexus. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Human Leptin R by Western Blot
Expression and isolation of recombinant murine LepRec-Fc fusion proteins.a) Expression of murine LepRec-Fc constructs in adherent HEK293T/17 cells. Supernatants containing LepRec-Fc chimera proteins from 24 and 48 hours post-transfection were cleared of cellular debris, subjected to SDS PAGE and western blotted with antibodies against domains of murine IgG1 (Fc specific for lanes 1–5 and Ab specific for lanes 6–8). Lane 1: Mock transfected at 48 hours. Lane 2: WT transfected at 24 hours. Lane 3: WT transfected at 48 hours. Lane 4: Q223R transfected at 24 hours. Lane 5: Q223R transfected at 48 hours. Lane 6: Mock transfected at 48 hours. Lane 7: WT transfected at 48 hours. Lane 8: Q223R transfected at 48 hours. b) Concentration and buffer exchange of murine LepRec-Fc chimeras. Supernatants were collected 48 hours after growth medium was replaced with expression medium (serum free Optimem +2 mM sodium butyrate). These supernatant preparations were concentrated by Amicon ultrafiltration (NMWCO of 100 kDa). Supernatants, concentrates, and filtrates from each chimera were subjected to SDS-PAGE followed by coomassie staining (top) and western blotting with alpha-murine IgG1 specific to the Fc region (bottom). Lane 1: Expression medium. Lane 2: WT supernatant. Lane 3: WT concentrate. Lane 4: WT filtrate. Lane 5: Q223R supernatant. Lane 6: Q223R Concentrate. Lane 7: Q223R Filtrate. c) Supernatants, concentrates, and filtrates (as in b) from each chimera were subjected to SDS-PAGE followed by transfer to PVDF membrane and western blotting with alpha-murine leptin receptor (R&D scientific). Lane 1: Expression medium. Lane 2: WT supernatant. Lane 3: WT concentrate. Lane 4: WT filtrate. Lane 5: Q223R Filtrate. Lane 6: Q223R Concentrate. Lane 7: Q223R supernatant. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24743494), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse Leptin R Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain
Sample: Perfusion fixed frozen sections of rat brain
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR)
Sample: Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using AF497 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Leptin R
Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R has recently been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.
Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R has been shown to bind leptin with high affinity and is a potent leptin antagonist.
References
- Tartaglia, L.A. et al. (1995) Cell 83:1263.
- Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
- Lee, J.I. and J.M. Friedman (1996) Nature 379:632.
- Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
- Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.
Long Name
Leptin Receptor
Alternate Names
B219, CD295, LEPR, LeptinR, OB R
Gene Symbol
LEPR
UniProt
Additional Leptin R Products
Product Documents for Mouse Leptin R Antibody
Product Specific Notices for Mouse Leptin R Antibody
For research use only
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