Mouse Lymphotoxin beta R/TNFRSF3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1008
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Cited:
Bioassay, Flow Cytometry, Functional Assay, Immunohistochemistry-Frozen, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Lymphotoxin betaR/TNFRSF3
Ser28-Pro218
Accession # P50284
Ser28-Pro218
Accession # P50284
Specificity
Detects mouse Lymphotoxin betaR/TNFRSF3 in direct ELISA and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) LT beta R is observed, and less than 1% cross-reactivity with recombinant mouse (rm) 4-1BB, rmCD27, rmOPG, rmRANK, rmCD30, rmCD40, rhDR6, rmTNF RI, rmTNF RII, rmEDAR, rmFas and rmGITR is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Lymphotoxin beta R/TNFRSF3 Antibody
Detection of Lymphotoxin betaR/TNFRSF3 in NIH‑3T3 Mouse Cell Line by Flow Cytometry.
NIH-3T3 mouse embryonic fibroblast cell line was stained with Goat Anti-Mouse Lymphotoxin betaR/TNFRSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1008, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse Lymphotoxin beta R/TNFRSF3 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: NIH‑3T3 mouse embryonic fibroblast cell line
Sample: NIH‑3T3 mouse embryonic fibroblast cell line
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus
Sample: Perfusion fixed frozen sections of mouse thymus
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Lymphotoxin betaR/TNFRSF3
Sample: Recombinant Mouse Lymphotoxin betaR/TNFRSF3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lymphotoxin beta R/TNFRSF3
Long Name
Lymphotoxin beta Receptor
Alternate Names
LTBR, LymphotoxinbR, TNF RIII, TNF Rrp, TNFRSF3
Entrez Gene IDs
Gene Symbol
LTBR
UniProt
Additional Lymphotoxin beta R/TNFRSF3 Products
Product Documents for Mouse Lymphotoxin beta R/TNFRSF3 Antibody
Product Specific Notices for Mouse Lymphotoxin beta R/TNFRSF3 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...