Mouse M-Cadherin/Cadherin-15 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7677
Key Product Details
Species Reactivity
Mouse
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 800516
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse M-Cadherin/Cadherin-15
Val22-Ala605
Accession # P33146
Val22-Ala605
Accession # P33146
Specificity
Detects mouse M-Cadherin/Cadherin-15 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human M-Cadherin, recombinant mouse (rm) Cadherin-13, or rmP-Cadherin is
observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse M-Cadherin/Cadherin-15 Antibody
M‑Cadherin/Cadherin‑15 in C2C12 Mouse Cell Line.
M-Cadherin/Cadherin-15 was detected in immersion fixed C2C12 mouse myoblast cells differentiated for 5 days with 5% equine serum using Rat Anti-Mouse M-Cadherin/Cadherin-15 Monoclonal Antibody (Catalog # MAB7677) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of developing myotubes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse M-Cadherin/Cadherin-15 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line differentiated for 5 days with 5% equine serum
Sample: Immersion fixed C2C12 mouse myoblast cell line differentiated for 5 days with 5% equine serum
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: M-Cadherin/Cadherin-15
CDH-15 (Cadherin 15; also M-cadherin, Muscle cadherin and Cadherin-14) is a 125-127 kDa atypical member of the classical cadherin family, cadherin superfamily of molecules. It is expressed by muscle satellite cells, cells of the embryonic myotome, and hematopoietic bone marrow stem cells. CDH-15 appears to bind homotypically in trans, thus allowing for the identification and subsequent fusion of myoblast precursors, particularly those in slow-twitch (or red fiber) muscles. This is accompanied by a downregulation of mitochondrial induced apoptosis. Mouse CDH-15 is synthesized as a 784 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, a 38 aa propeptide, and a 725 aa mature region. The mature region is expressed as a type I transmembrane glycoprotein that possesses a 546 aa extracellular region (aa 60-605) and a 159 aa cytoplasmic domain (aa 626-784). The extracellular region shows five consecutive cadherin domains. Over aa 22-605, mouse CDH-15 shares 88% and 97% aa sequence identity with human and rat CDH-15, respectively.
Long Name
M-Cadherin [Myotubule]
Alternate Names
Cadherin-15, CDH14, CDH15, CDHM, MCAD, MCadherin
Gene Symbol
CDH15
UniProt
Additional M-Cadherin/Cadherin-15 Products
Product Documents for Mouse M-Cadherin/Cadherin-15 Antibody
Product Specific Notices for Mouse M-Cadherin/Cadherin-15 Antibody
For research use only
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